Comparison of nutritional components (isoflavone, protein, oil, and fatty acid) and antioxidant properties at the growth stage of different parts of soybean [Glycine max (L.) Merrill] (2025)

. 2017 Apr 30;26(2):339–347. doi: 10.1007/s10068-017-0046-x

Woo Duck Seo

², Jae Eun Kang

¹, Sik-Won Choi

², Kwang-Sik Lee

², Mi-Ja Lee

², Ki-Do Park

², Jin Hwan Lee

^1,^✉

PMCID: PMC6049444PMID: 30263548

Abstract

This is the first study to investigate antioxidant capacities of isoflavones prepared using microwave-assisted hydrolysis method from different parts (seeds, leaves, leafstalks, pods, stems and roots) of soybean at growth stages. In addition, the fluctuations in the isoflavone, protein, fatty acid, and oil contents in R6-R8 (R6: beginning; R7: beginning maturity; R8: full maturity) seeds were confirmed. The R7 seeds exhibited the most predominant contents of isoflavones (1218.1±7.3 μg/g) in the following order: daidzein (48%)>genistein (35%)>glycitein (17%). The second highest isoflavone content was found in the leaves (1052.1±10.4 μg/g), followed by R8 seeds>roots>R6 seeds>leafstalks> pods; the stems exhibited the lowest isoflavone content (57.2±1.7 μg/g). Interestingly, daidzein showed the highest individual isoflavone content with remarkable variations (57.2-766.8 μg/g), representing 46-100% of the total isoflavone content. R8 exhibited higher protein, fatty acid, and oil contents than R6 or R7. Moreover, the antioxidant capacities against two radicals in different parts of soybean plant showed considerable differences depending upon the isoflavone content. Our results suggested that soybean leaves and seeds might be useful materials for functional foods.

Keywords: soybean seeds, soybean leaves, isoflavone, antioxidant activity, growth stage

References

1.Chen KI, Erh MH, Su NW, Liu WH, Chou CC. Soy foods and soybean product: From traditional use to modern applications. Appl. Microbiol. Biot. 2012;96:9–22. doi: 10.1007/s00253-012-4330-7. [DOI] [PubMed] [Google Scholar]
2.Cho KM, Ha TJ, Lee YB, Seo WD, Kim JY, Ryu HW, Jeong SH, Kang YM, Lee J H. Soluble phenolics and antioxidant properties of soybean (Glycine max L.) cultivars with varying seed coat colours. J. Funct. Food. 2013;5:1065–1076. doi: 10.1016/j.jff.2013.03.002. [DOI] [Google Scholar]
3.Lee JH, Lee BW, Kim JH, Jeong TS, Kim MJ, Lee WS, Park KH. LDL-antioxidant pterocarpans from roots of Glycine max (L.) Merr. J. Agr. Food Chem. 2006;54:2057–2063. doi: 10.1021/jf052431c. [DOI] [PubMed] [Google Scholar]
4.Lee JH, Cho KM. Changes occurring in compositional components of black soybeans maintained at room temperature for different storage periods. Food Chem. 2012;131:161–169. doi: 10.1016/j.foodchem.2011.08.052. [DOI] [Google Scholar]
5.Li Y, Liang X, Zhang Y, Gao J. Effects of different dietary soybean oil levels on growth, lipid deposition, tissues fatty acid composition and hepatic lipid metabolism related gene expressions in blunt snout bream (Megalobrama amblycephala) juvenile. Aquaculture. 2016;451:16–23. doi: 10.1016/j.aquaculture.2015.08.028. [DOI] [Google Scholar]
6.Han T, Wu C, Tong Z, Mentreddy RS, Tan K, Gai J. Postflowering photoperiod regulates vegetative growth and reproductive development of soybean. Environ. Exp. Bot. 2006;55:120–129. doi: 10.1016/j.envexpbot.2004.10.006. [DOI] [Google Scholar]
7.Kim JA, Chung IM. Change in isoflavone concentration of soybean (Glycine max L.) seeds at different growth stages. J. Sci. Food Agr. 2007;87:496–503. doi: 10.1002/jsfa.2743. [DOI] [Google Scholar]
8.Kumar V, Rani A, Dixit AK, Bhatnagar D, Chauhan GS. Relative changes in tocopherols, isoflavones, total phenolic content, and antioxidative activity in soybean sees at different reproductive stages. J. Agr. Food Chem. 2009;57:2705–2710. doi: 10.1021/jf803122a. [DOI] [PubMed] [Google Scholar]
9.Antony MS, Clarkson TB, Hughes CL, Morgan TM, Burke GL. Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkeys. J. Nutr. 1996;126:43–50. doi: 10.1093/jn/126.1.43. [DOI] [PubMed] [Google Scholar]
10.Scheiber MD, Liu JH, Subbiah MTR, Rebar RW, Setchell KDR. Dietary inclusion of whole soy foods results in significant reductions in clinical risk factors for osteoporosis and cardiovascular disease in normal postmenopausal women. Menopause. 2001;8:384–392. doi: 10.1097/00042192-200109000-00015. [DOI] [PubMed] [Google Scholar]
11.Lee JH, Hwang CW, Lee BW, Kim HT, Ko JM, Baek IY, Ahn MJ, Lee HY, Cho KM. Effects of roasting on the phytochemical contents and antioxidant activities of Korean soybean (Glycine max L. Merrill) cultivars. Food Sci. Biotechnol. 2015;24:1573–1582. doi: 10.1007/s10068-015-0203-z. [DOI] [Google Scholar]
12.Piskula MK, Yamakoshi J, Iwai Y. Daidzein and genistein but not their glucosides are absorbed from the rat stomach. FEBS Lett. 1999;447:287–291. doi: 10.1016/S0014-5793(99)00307-5. [DOI] [PubMed] [Google Scholar]
13.Izumi T, Piskula MK, Osawa S, Obata A, Saito M, Kataoka S, Kubota Y, Kikuchi M. Soybean isoflavone aglycones are absorbed faster and in higher amounts than their glucosides. J. Nutr. 2000;130:1695–1699. doi: 10.1093/jn/130.7.1695. [DOI] [PubMed] [Google Scholar]
14.Wang M, Guo J, Qi W, Su R, He Z. An effective and green method for the extraction and purification of aglycone isoflavones from soybean. Food Sci. Biotechnol. 2013;22:705–712. doi: 10.1007/s10068-013-0135-4. [DOI] [Google Scholar]
15.Lee JH, Choung MG. Determination of optimal acid hydrolysis time of soybean isoflavones using drying oven and microwave assisted methods. Food Chem. 2011;129:577–582. doi: 10.1016/j.foodchem.2011.04.069. [DOI] [PubMed] [Google Scholar]
16.Chatterrjee D, Bhattacharjee P. Comparative evaluation of the antioxidant efficacy of encapsulated and un-encapsulated eugenol-rich clove extracts in soybean oil: Shelf-life and frying stability of soybean oil. J. Food Eng. 2013;117:545–550. doi: 10.1016/j.jfoodeng.2012.11.016. [DOI] [Google Scholar]
17.Rayaprolu SJ, Hettiarachchy NS, Chen P, Kannan A, Mauromostakos A. Peptides derived from high oleic acid soybean meals inhibit colon, liver and lung cancer cell growth. Food Res. Int. 2013;50:282–288. doi: 10.1016/j.foodres.2012.10.021. [DOI] [Google Scholar]
18.Yuan B, Ren J, Zhao M, Luo D, Gu L. Effects of limited enzymatic hydrolysis with pepsin and high-pressure homogenization on the functional properties of soybean protein isolate. LWT-Food Sci. Technol. 2012;46:453–459. doi: 10.1016/j.lwt.2011.12.001. [DOI] [Google Scholar]
19.Lee JH, Park KH, Lee MH, Kim HT, Seo WD, Kim JY, Baek IY, Jang DS, Ha TJ. Identification, characterization, and quantification of phenolic compounds in the antioxidant activity-containing fraction from the seeds of Korean perilla (Perilla frutescens) cultivars. Food Chem. 2013;136:843–852. doi: 10.1016/j.foodchem.2012.08.057. [DOI] [PubMed] [Google Scholar]
20.Choi Y, Jeong SH, Lee J. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem. 2007;103:130–138. doi: 10.1016/j.foodchem.2006.08.004. [DOI] [Google Scholar]
21.Seol NG, Ka HJ, Yi BR, Song JH, Park JH, Jung JY, Kim MJ, Lee JH. Changes in isoflavone profiles and antioxidant activities in isoflavone extracts from soybeans and soy foods under riboflavin photosensitization. Food Sci. Biotechnol. 2015;24:1271–1277. doi: 10.1007/s10068-015-0163-3. [DOI] [Google Scholar]
22.Magarelli G, Lima L d, Silva JG, SouzaDe JR, de Castro CSP. Rutin and total isoflavone determination in soybean at different growth stages by using voltammetric methods. Microchem. J. 2014;117:149–155. doi: 10.1016/j.microc.2014.06.019. [DOI] [Google Scholar]
23.Simirgiotis MJ, Caligari PDS, Schmeda-Hirschmann G. Identification of phenolic compounds from the fruits of the mountain papaya Vasconcellea pubescens A. DC. grown in Chile by liquid chromatography-UV detection-mass spectrometry. Food Chem. 2009;115:775–784. [Google Scholar]
24.Sakthivelu G, Devi MKA, Giridhar P, Rajasekaran T, Ravishankar GA, Nikolova MT, Angelov GB, Todorova RM, Kosturkova GP. Isoflavone composition, phenol content, and antioxidant activity of soybean seeds from India and Bulgaria. J. Agr. Food Chem. 2008;56:2090–2095. doi: 10.1021/jf072939a. [DOI] [PubMed] [Google Scholar]
25.Wu J, Muir AD. Isoflavone during protease hydrolysis of defatted soybean meal. Food Chem. 2010;118:328–332. doi: 10.1016/j.foodchem.2009.04.129. [DOI] [Google Scholar]
26.Brummer EC, Graef GL, Orf J, Wilcox JR, Shoemaker RC. Mapping QTL for seed protein and oil content in eight soybean populations. Crop Sci. 1997;37:370–378. doi: 10.2135/cropsci1997.0011183X003700020011x. [DOI] [Google Scholar]
27.Cosio MS, Ballabio D, Benedetti S, Gigliotti C. Evaluation of different storage conditions of extra virgin olive oils with an innovative recognition tool built by means of electronic nose and electronic tongue. Food Chem. 2007;101:485–491. doi: 10.1016/j.foodchem.2006.02.005. [DOI] [Google Scholar]
28.Shewry PR, Napier JA, Tatham AS. Seed storage proteins: Structures and biosynthesis. Plant Cell. 1995;7:945–956. doi: 10.1105/tpc.7.7.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
29.Kumar V, Rani A, Dixit AK, Pratap D, Bhatnagar D. A comparative assessment of total phenolic content, ferric reducing-anti-oxidative power, free radicalscavenging activity, vitamin C and isoflavones content in soybean with varying seed coat colour. Food Res. Int. 2010;43:323–328. doi: 10.1016/j.foodres.2009.10.019. [DOI] [Google Scholar]
30.Herchi W, Bouali I, Bahashwan S, Rochut S, Boukhchina S, Kallel H, Pepe C. Changes in phospholipid composition, protein content and chemical properties of flaxseed oil during development. Plant Physiol. Bioch. 2012;54:1–5. doi: 10.1016/j.plaphy.2012.02.008. [DOI] [PubMed] [Google Scholar]
31.Akitha Devi MK, Gondi M, Sakthivelu G, Giridhar P, Rajasekaran T, Ravishankar GA. Functional attributes of soybean seeds and products, with reference to isoflavone content and antioxidant activity. Food Chem. 2009;114:771–776. doi: 10.1016/j.foodchem.2008.10.011. [DOI] [Google Scholar]
32.Niki E. Assessment of antioxidant capacity in vitro and in vivo. Free Radical Bio. Med. 2010;49:503–515. doi: 10.1016/j.freeradbiomed.2010.04.016. [DOI] [PubMed] [Google Scholar]
33.Alu’datt MH, Rababah T, Ereifej K, Alli I. Distribution, antioxidant and characterisation of phenolic compounds in soybeans, flaxseed and olives. Food Chem. 2013;139:93–99. doi: 10.1016/j.foodchem.2012.12.061. [DOI] [PubMed] [Google Scholar]
34.Viuda-Martos M E-E, Gendy A, Sendra E, Fernandez-Lopez J E, Razik K A, Omer EA, Perez-Alvarez JA. Chemical composition, antioxidant and anti-Listeria activity of essential oils obtained from some Egyptian plants. J. Agr. Food Chem. 2010;58:9063–9070. doi: 10.1021/jf101620c. [DOI] [PubMed] [Google Scholar]
35.Lee JH, Baek IY, Choung MG, Ha TJ, Han WY, Cho KM, Jeong SH, Oh KW, Park KY, Park KH. Phytochemical constituents from the leaves of soybean [Glycine max (L.) Merr.] Food Sci. Biotechnol. 2008;17:578–586. [Google Scholar]